Statistical image analysis reveals features affecting fates of Myxococcus xanthus developmental aggregates
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چکیده
منابع مشابه
Statistical image analysis reveals features affecting fates of Myxococcus xanthus developmental aggregates.
Starving Myxococcus xanthus bacteria use their motility systems to self-organize into multicellular fruiting bodies, large mounds in which cells differentiate into metabolically inert spores. Despite the identification of the genetic pathways required for aggregation and the use of microcinematography to observe aggregation dynamics in WT and mutant strains, a mechanistic understanding of aggre...
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A set of developmental mutants have been examined that behave as if defective in cellular interactions necessary for the formation of myxospores during fruiting body development. Sporulation is rescued in these mutants if they are mixed with wild-type cells. Complementation experiments with whole cells divide the mutants into four groups (A, B, C, and D). Mutants of group A appear to be less re...
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Myxococcus xanthus genetic mutants with characterized phenotypes were analysed for the ability to prey on susceptible bacteria. Quantification of predatory ability was scored by a newly developed method under conditions in which prey bacteria provided the only source of nutrients. These results were corroborated by data derived using a previously published protocol that measures predation in th...
متن کاملDisruption of aldA influences the developmental process in Myxococcus xanthus.
Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA does encode a functional alanine dehydrogenase, since it catalyzes the reversi...
متن کاملDevelopmental bypass suppression of Myxococcus xanthus csgA mutations.
The csgA mutations of Myxococcus xanthus (formerly known as spoC) inhibit sporulation as well as rippling, which involves ridges of cells moving in waves. Sporulating revertants of CsgA cells were isolated by direct selection, since spores are much more resistant to heat and ultrasonic treatment than are vegetative cells. The revertants fell into seven groups on the basis of phenotype and the c...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2011
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1018383108